Using cutting-edge genomic tools, members of our lab investigate population genomics and marine population connectivity in a variety of organisms from fish like the Atlantic salmon (Salmo salar) and Atlantic cod (Gadus morhua) to invertebrates like the green crab (Carcinus maenas) and the sea scallop (Placopecten magellanicus).
One of the main tools we use to generate high-quality genomic data is RAD-seq (restriction site associated DNA sequencing) in conjunction with Illumina high-throughput sequencing. In brief, the method involves cutting a genome with at least one restriction enzyme and sequencing the ends of the resulting fragments using an Illumina Genome Analyzer. Fragments can be barcoded to allow the pooling of individuals into a single lane. This enables the identification, mapping and genotyping of 1000’s of SNPs easily and cheaply. Proposed RAD tag genome scan projects include wild and farmed Atlantic salmon, invasive green crab in eastern Canada, and thermal adaptation in Atlantic Sea Scallop. For more info
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